Template Switch Oligo
Template Switch Oligo - Use this set of oligonucleotides for template switching cdna synthesis and preamplification. Template switching oligonucleotide and capturing oligo (dt) are used to incorporate adaptor. The tso (template switch oligo) is an oligo that hybridizes to untemplated c nucleotides added by the reverse transcriptase during reverse transcription. We recommend a tso with rgrgrg at the 3′ end. However, if a large fraction of the library contains a portion. The resulting cdna can be amplified by pcr or serve as.
A small fraction of visium libraries are expected to contain the template switching oligo (tso) at the beginning of read 2. We recommend a tso with rgrgrg at the 3′ end. The resulting cdna can be amplified by pcr or serve as. In general, different tsos can be used including tsos with 5′ modifications, such as biotin, isomeric bases or abasic spacers. Template switching oligonucleotide and capturing oligo (dt) are used to incorporate adaptor.
Template Switch Oligo
In general, different tsos can be used including tsos with 5′ modifications, such as biotin, isomeric bases or abasic spacers. The resulting cdna can be amplified by pcr or serve as. The tso (template switch oligo) is an oligo that hybridizes to untemplated c nucleotides added by the reverse transcriptase during reverse transcription. We recommend a tso with rgrgrg at.
Template Switch Oligo
In conjunction with a template switching oligo (tso), cdna is synthesized with a known sequence of choice attached to the 3′ end. Use this set of oligonucleotides for template switching cdna synthesis and preamplification. (step 2) mmlv reverse transcriptase adds deoxycytosines to the cdna 3' end. We recommend a tso with rgrgrg at the 3′ end. The tso (template switch.
Template Switch Oligo Printable Word Searches
(step 2) mmlv reverse transcriptase adds deoxycytosines to the cdna 3' end. A small fraction of visium libraries are expected to contain the template switching oligo (tso) at the beginning of read 2. We recommend a tso with rgrgrg at the 3′ end. In conjunction with a template switching oligo (tso), cdna is synthesized with a known sequence of choice.
Template Switch Oligo Printable Word Searches
The tso adds a common 5'. The resulting cdna can be amplified by pcr or serve as. We recommend a tso with rgrgrg at the 3′ end. However, if a large fraction of the library contains a portion. Template switching oligonucleotide and capturing oligo (dt) are used to incorporate adaptor.
Template Switch Oligo
The tso adds a common 5'. (step 2) mmlv reverse transcriptase adds deoxycytosines to the cdna 3' end. However, if a large fraction of the library contains a portion. In conjunction with a template switching oligo (tso), cdna is synthesized with a known sequence of choice attached to the 3′ end. Use this set of oligonucleotides for template switching cdna.
Template Switch Oligo - The tso adds a common 5'. In conjunction with a template switching oligo (tso), cdna is synthesized with a known sequence of choice attached to the 3′ end. Template switching oligonucleotide and capturing oligo (dt) are used to incorporate adaptor. The tso (template switch oligo) is an oligo that hybridizes to untemplated c nucleotides added by the reverse transcriptase during reverse transcription. We recommend a tso with rgrgrg at the 3′ end. The resulting cdna can be amplified by pcr or serve as.
Use this set of oligonucleotides for template switching cdna synthesis and preamplification. In conjunction with a template switching oligo (tso), cdna is synthesized with a known sequence of choice attached to the 3′ end. The tso (template switch oligo) is an oligo that hybridizes to untemplated c nucleotides added by the reverse transcriptase during reverse transcription. Template switching oligonucleotide and capturing oligo (dt) are used to incorporate adaptor. The tso adds a common 5'.
The Tso (Template Switch Oligo) Is An Oligo That Hybridizes To Untemplated C Nucleotides Added By The Reverse Transcriptase During Reverse Transcription.
The tso adds a common 5'. Use this set of oligonucleotides for template switching cdna synthesis and preamplification. We recommend a tso with rgrgrg at the 3′ end. A small fraction of visium libraries are expected to contain the template switching oligo (tso) at the beginning of read 2.
In General, Different Tsos Can Be Used Including Tsos With 5′ Modifications, Such As Biotin, Isomeric Bases Or Abasic Spacers.
Template switching oligonucleotide and capturing oligo (dt) are used to incorporate adaptor. The resulting cdna can be amplified by pcr or serve as. In conjunction with a template switching oligo (tso), cdna is synthesized with a known sequence of choice attached to the 3′ end. (step 2) mmlv reverse transcriptase adds deoxycytosines to the cdna 3' end.